8,9-anellated-1,2,3,4-tetrahydro-β-carboline derivatives

ABSTRACT

New 8,9-annelated-1,2,3,4-tetrahydro-β-carboline derivatives of the formula ##STR1## wherein R 1  -R 7  are as defined herein; and pharmaceutically acceptable salts and prodrugs thereof. A method of dissolving blood clots using this compound and a pharmaceutical composition having fibrinolytic activity comprising this compound are also part of the present invention.

This is a division of application Ser. No. 07/428,481, filed Oct. 30, 1989, now U.S. Pat. No. 5,100,884, which in turn is a continuation of application Ser. No. 07/219,362, filed Jul. 15, 1988, now abandoned.

The invention relates to a group of new 8,9-anellated-1,2,3,4-tetrahydro-β-carboline derivatives and salts and prodrugs thereof, to the preparation of these compounds and to pharmaceutical compositions which comprise at least one of these compounds as an active substance.

It has been found surprisingly that the compounds of formula 1 ##STR2## and the salts and prodrugs thereof have good fibrinolytic properties, and in particular may be used as orally active fibrinolytics.

The symbols in the above formula 1 have the following meanings:

R₁ is straight or branched alkyl having 1-6 C-atoms, fluorinated or hydroxylated alkyl having 1-6 C-atoms, phenylalkyl having 1-6 C-atoms in the alkyl group, the phenyl group of which may optionally be substituted, or two alkyl groups R₁ bonded to adjacent carbon atoms together constitute a ring of 5-7 carbon atoms, which ring may be substituted with alkyl groups having 1-3 C-atoms, or R₁ is straight or branched alkenyl or alkynyl having 2-6 C-atoms, which groups may comprise one or more fluorine atoms, or cycloalkyl having 3-6 C-atoms which are substituted with 0-4 methyl groups, or R₁ is straight or branched alkoxy or alkylthio having 1-6 C-atoms which may be substituted with one or more fluorine atoms or hydroxyl groups, or with one optionally substituted phenyl group, or two alkoxy groups and/or alkylthio groups bonded to two adjacent carbon atoms may form a ring consisting of 5-7 ring atoms which may be substituted with hydroxymethyl, alkoxy or alkoxyalkyl groups having 1-6 C-atoms, or R₁ is a cycloalkoxy group or cycloalkylthio group having 3-6 C-atoms which may be substituted with 1-4 methyl groups, or R₁ is straight or branched alkoxy-, alkylthio- or alkylsulphonylalkyl having 2-6 C-atoms which may comprise one or more fluorine atoms or hydroxyl groups, or R₁ is an alkoxycarbonylmethyl group having 1-6 C-atoms in the alkoxy group, or R₁ is a group R₈ R₉ N--CO--CH₂ --, R₈ R₉ N--CO-- or R₈ R₉ N--SO₂ wherein R₈ and R₉ independently of each other are hydrogen, alkyl having 1-3 C-atoms, or, together with the nitrogen atom, form a heterocyclic 5- or 6-ring, or R₁ is hydroxy, halogen, cyano, straight or branched alkoxycarbonyl having 1-6 C-atoms in the alkoxy group, cycloalkylsulphonyl having 3-8 C-atoms which may be substituted with 1-4 methyl groups; n has the value 0-3;

R₂ +R₃ together with the carbon atom and the nitrogen atom to which they are bonded and the intermediate carbon atom constitute a heterocyclic group consisting of 5-8 ring atoms which, in addition to the nitrogen atom already present, may comprise a second hetero atom from the group N, O, S, S--O or SO₂, and which may be substituted with 1-3 alkyl groups having 1-4 C-atoms which can form a spiroalkyl group having 2-5 C-atoms, or the hetero group may be substituted with a phenyl group comprising 0-3 groups R₁₀, wherein R₁₀ is alkyl or alkoxy having 1-3 C-atoms, halogen, trifluoromethyl, cyano or hydroxy and two alkyl groups or alkoxy groups R₁₀ bonded to adjacent carbon atoms may form a ring anellated with the phenyl group and consisting of 5-7 ring atoms, which ring may be substituted with alkyl or alkoxy having 1-3 C-atoms, or with akoxyalkyl having 1-6 C-atoms, and the ring formed by R₂ +R₃ may be anellated with a saturated or unsaturated carbocyclic or heterocyclic ring consisting of 5- or 6-ring atoms which may be substituted with halogen or alkyl having 1-4 C-atoms;

R₄ is hydrogen, straight or branched alkyl having 1-8 C-atoms, alkoxy- or alkylthioalkyl having 2-6 C-atoms, alkenyl or alkynyl having 2-8 C-atoms, which groups may be substituted with one or more fluorine atoms or hydroxy groups, or with one phenyl group containing 0-3 groups R₁₀, or cycloalkyl group having 3-7 C-atoms, or R₄ is cycloalkyl having 3-8 C-atoms which may be substituted with one or more fluorine atoms, alkyl groups having 1-4 C-atoms, cycloalkyl groups having 3-5 C-atoms, or R₄ is cycloalkenyl having 5-7 C-atoms which may be substitutued with 1-4 methyl groups, or R₄ is straight or branched alkoxycarbonylalkyl having 0-6 C-atoms in the alkoxy group and 1-3 C-atoms in the alkyl group, or R₄ is a group R₈ R₉ N--CO--R₁₁ -- or R₈ R₉ N--SO₂ --R₁₁ --, wherein R₈ and R₉ have the above-mentioned meanings and R₁₁ is alkyl having 1-3 C-atoms, or R₄ is alkylsulphonylalkyl having 1-3 C-atoms per alkyl group, or a phenylsulphonylalkyl group having 1-3 C-atoms in the alkyl group and the phenyl group comprises 0-3 groups R₁₀, or R₄ is a phenyl group substituted with 0-4 groups R₁₂, wherein R₁₂ is straight or branched alkyl having 1-6 C-atoms which may be substituted with one or more fluorine atoms or hydroxyl groups, or with one cyano group, or with straight or branched alkoxycarbonyl having 0-6 C-atoms in the alkoxy group, or with a group R₈ R₉ N--CO-- or R₈ R₉ N--SO₂ --, wherein R₈ and R₉ have the above-mentioned meanings, or two groups R₁₂ bonded to adjacent carbon atoms form a ring anellated with the phenyl group and consisting of 5-7 ring atoms, which ring may be substituted with 1-3 alkyl groups having 1-3 C-atoms, or R₁₂ is straight or branched alkyl (1-4 C)-oxy-alkyl(0-3 C), alkyl (1-4 C)-thio-alkyl(0-3 C) or alkyl(1-4 C)-sulphonylalkyl(0-3 C) which groups may comprise one or more fluorine atoms or hydroxyl groups, or of which two alkoxy groups or alkylthio groups bonded to adjacent carbon atoms form a ring which consists of 5-7 ring atoms and may be substituted with alkyl or alkoxy having 1-3 C-atoms, or with hydroxymethyl, or with alkoxyalkyl having 2-6 C-atoms, or R₁₂ is cycloalkoxy or cycloalkylthio having 3-7 C-atoms which may be substituted with 0-4 methyl groups, or R₁₂ is cycloalkyl having 3-7 C-atoms in which one or more fluorine atoms or methyl groups may be present, or R₁₂ is straight or branched alkoxycarbonyl having 1-6 C-atoms in the alkoxy group, a groups R₈ R₉ N--CO-- or R₈ R₉ N--SO₂ -- wherein R₈ and R₉ have the above-mentioned meanings, or R₁₂ is halogen or hydroxy, or R₄ is mono- or bicyclic heteroaryl group in which N and/or O and/or S may be present as hetero atoms, and the ring(s) may be substituted with alkyl, alkoxy or alkylthio having 1-3 C-atoms, halogen, keto oxygen atom, cyano and/or CF₃ ;

R₅ is hydrogen or straight or branched alkyl having 1-6 C-atoms; or

R₄ +R₅, together with the carbon atom to which they are bonded, may form a carbocyclic or heterocyclic ring consisting of 5-7 ring atoms which may comprise N, O, S, SO or SO₂ as a hetero atom;

R₆ is hydrogen, straight or branched alkyl having 1-6 C-atoms which may be substituted with one or more hydroxyl groups or with one optionally substituted phenyl group or alkoxycarbonyl group having 1-3 C-atoms in the alkoxy group, or R₆ is a straight or branched alkenyl or alkynyl having 2-6 C-atoms, or a group --CO--X, wherein X is straight or branched alkyl, alkenyl, alkynyl or alkoxy having 1-6 C-atoms, or an optionally substituted phenyl group, or R₆ is an amino acid radical of the formula --CO--CHR₁₃ --NH--R₁₄, wherein R₁₃ is alkyl and R₁₄ is hydrogen, alkanoyl or alkoxycarbonyl having 1-4 C-atoms;

R₇ is alkyl having 1-3 C-atoms, or one group R₇ is an alkoxycarbonyl group having 1-3 C-atoms in the alkoxy group, or the hydroxymethyl group; and

p has the value 0-4.

Suitable acids with which the compounds of formula 1 according to the invention can form pharmaceutically acceptable acid addition salts are, for example, hydrochloric acid, sulphuric acid, phosphoric acid, nitric acid, and organic acids, for example, citric acid, fumaric acid, maleic acid, tartaric acid, acetic acid, benzoic acid, p-toluene sulphonic acid, methane sulphonic acid, and the like.

When the symbols R₄ and R₅ have different meanings and/or when p has the value 1, 2 or 3 and/or when R₂ and R₃, together with the carbon atom and nitrogen atom, respectively, to which they are bonded and the intermediate carbon atom form a ring which is substituted, the compounds of formula 1 comprise one or more chiral centres. The invention relates both to racemates and individual enantiomers.

The invention also relates to prodrugs of the compounds of formula 1, i.e. derivatives of these compounds which as such are inactive, from which, after splitting off of an easily removable group, for example an ester group or an ether group, an active compound of formula 1 is obtained.

The carboline derivatives according to the invention are orally active fibrinolytics and may hence be used in the control of already formed venous or arterial thrombi or may be administered to prevent thrombi. The compounds may be used, for example, for a short period of time in operations, or for long periods in the case of enhanced risk after, for example, myocard infarct, cerebral or peripheral suffering. The best compounds probably are active via an increase of the tissue plasminogen activator activity as a result of which the possibility of spontaneous bleedings can be prevented.

The compounds according to the invention are fibrinolytically active in oral doses of less than 50 mg/kg.

The oral fibrinolytic activity of the compounds according to the invention was established first of all in rats in the so-called DBCLT (Diluted Blood Clot Lysis Test; Taylor F. B. et al, Fed. Proc. (1981), 40 (2092-2098). Rats are treated orally with the test compound. After 1-3 hours blood is taken. ¹²⁵ I-labelled fibrinogen and thrombine are added so that a blood clot is formed which, depending on the extent of fibrinolytic activity caused by the test compound dissolves more rapidly as compared with blood clots of untreated animals.

The increase of tissue plasminogen activator activity was measured in cultures of endothelinm cells (Thrombosis Diathesis Haemorrhagis (Stuttgart), 34, (1975), pp. 825-839; and Thrombosis and Haemostasis, 51, (1984), p. 392).

Concentrations of less than 10 /umol per liter of the compounds give a good increase of the plasminogen activator activity.

A few of the compounds falling within the scope of the present invention have a psychotropic activity, for example, antiaggressive or antipsychotic. These activities have been found in the tests mentioned in the Netherlands Patent Applications 8403917 and 8403918.

The pharmacologically active compounds falling within the scope of the present invention, their prodrugs and salts can be brought into forms suitable for administration, for example, pills, tablets, coated tablets, capsules, powders, injection liquids, and the like, by means of techniques suitable for this purpose and while using suitable auxiliary substances, for example, solid or liquid carrier materials.

The dosage in which the compounds according to the invention may be used depend on the severity and the nature of the disease to be treated and on the way of administration.

The compounds of formula 1, dependently of the meanings of the symbols, may be prepared in at least one of the following manners

a) analogously to the method described in Advances in Heterocyclic Chemistry, Vol. 3. pp. 79-207 (The Carbolines). More in particular, compounds of this type can be obtained in a good yield by reaction of a compound of formula 2 ##STR3## wherein R₁ -R₃, n, p, R₆ and R₇ have the above-mentioned meanings, or a salt thereof, with a carbonyl compound of formula 3 ##STR4## wherein R₄ and R₅ have the above-mentioned meanings. The reaction is preferably carried out in a suitable solvent, for example, acetic acid, alcohol, etc., at a temperature between 10° and 120° C.

The starting compounds of formula 2 necessary for this mode of preparation can be obtained in a manner known for the synthesis of analogous compounds, for example, by reaction of a compound of formula 4 ##STR5## with a compound of formula 5 or 6 ##STR6## in which formulae R₁ -R₇, n and p have the above-mentioned meanings (see also Khimiya Geterotsiklicheskikh Soedineii, (1973), pp. 213-218 and J. Heterocyclic Chem. 11, (1974), pp. 387-393).

b) The compounds of formula 1 can also be obtained by reduction of a compound having formula 7 ##STR7## wherein R₁, n, R₂, R₃, R₄, R₇ and p have the above meanings with a suitable reducing agent, for example hydrogen and a catalyst.

The starting compounds of formula 7 can be obtained in good yield by means of the Bischler-Napieralsky ringclosure of a compound of the formula 8 ##STR8## wherein the symbols have the above given meanings. Suitable methods for this Bischler-Napieralsky reaction are described in Organic Reactions, vol. VI, page 74.

The compounds of formula 1 in which R₆ has a meaning other than hydrogen, can also be obtained by reacting the analogous compound wherein R₆ =H, in known manner with a compound R₆ -Y, wherein Y is a reactive group, for example, halogen, ethoxycarbonyloxy, or acetoxy.

The invention will now be described in greater detail with reference to the ensuing specific examples.

EXAMPLE I (R,S)-1-Cyclohexyl-1,2,3,4,9,10,11,12-octahydro-8H-pyrido[4',3':4,5]-pyrrolo-[3,2,1-kl]-[1]-benzazocine hydrochloride

a) 26.8 g (0.152 mol) of 1-amino-1,2,3,4,5,6-hexahydro-[1]-benzazocine were dissolved in a mixture of 400 ml of methanol and 40 ml of water, and the solution was heated to boiling. 17.8 g (0.167 mol) of 4-chlorobutanal were added carefully and the mixture was refluxed for 20 hours. The reaction mixture was then evaporated in vacuo and the residue was extracted with a mixture of methylene chloride and 2N sodium hydroxide. The organic layer was washed with water and evaporated in vacuo. The residue was purified by means of flash-chromatography on silicagel, using methylene chloride, methanol and ammonia (92.5:7:0.5) as an eluent. Yield 25.5 g (73%) of 1-(2-amino-ethyl)-5,6,7,8-tetrahydro-4H-pyrrolo-[3,2,1-kl]-[1]-benzazocine.

b) 30 g (0.132 mol) of 1-(2-amino-ethyl)-5,6,7,8-tetrahydro-4H-pyrrolo-[3,2,1-kl]-[1]-benzazocine were dissolved in 500 ml of acetic acid. 16.2 g (0.144 mol) of cyclohexane carboxaldehyde were added and the mixture was stirred at 40° C. for 70 hours. The mixture was then poured out on ice, made basic with 50% sodium hydroxide, and extracted with methylene chloride. The methylene chloride solution was washed with water and evaporated. The residue was purified by means of chromatography on silicagel using methylene chloride/methanol/ammonia (92.5:7:0.5) as an eluent. Yield 28 g (66%) of (R,S)-1-cyclohexyl-1,2,3,4,9,10,11,12-octahydro-8H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-kl]-[1]-benzazocine. The free base was dissolved in ethyl acetate and 1 equivalent of hydrochloric acid in ethanol was added. The solid substance was sucked off, washed and dried. The so-obtained hydrochloride had a melting point of 270.5°-271° C.

The compounds of formula 1 in table A hereinafter were obtained according to the methods of Example I:

    TABLE A       no. (R.sub.1).sub.n R.sub.2 +       R.sub.3 R.sub.4 R.sub.5 R.sub.6 (R.sub.7).sub.p salt m.p. °C.         1 5-Cl --CH.sub.2 CH.sub.2 -- cyclo-C.sub.6 H.sub.11 H H H HCl      268-271  2 6-Cl --CH.sub.2 CH.sub.2 -- cyclo-C.sub.6 H.sub.11 H H H base       82-83  3 7-Cl --CH.sub.2 CH.sub.2 -- benzyl H H H HCl 237-238  4 7-Cl      --CH.sub.2 CH.sub.2 -- H.sub.2 C═CF-- H H H HCl 105-110  5 H      --(CH.sub.2).sub.3 -- cyclo-C.sub.5 H.sub.9 H H H HCl 265.5-267.5  6 H      --(CH.sub.2).sub.3 -- phenyl H H 10-COOC.sub.2 H.sub.5 base * 104-110  7      H --(CH.sub.2).sub.3 -- cyclo-C.sub.8 H.sub.15 H H H HCl 252.5-253  8 H      --(CH.sub.2).sub.3 -- 4-CH.sub.3 -phenyl H H H HCl 280-283       9 H --(CH.sub.2).sub.3 -- 4-(CH.sub.3).sub.2 CH-phenyl H H H HCl      255-257 10 H --(CH.sub.2).sub.3 -- 4-(CH.sub.3).sub.2 CHO-phenyl H H H      HCl 178-180 11 H --C(CH.sub.3).sub.2 --(CH.sub.2).sub.2 -- cyclo-C.sub.6      H.sub.11 H H H HCl 281-283 12 H --C(CH.sub.3).sub.2 --(CH.sub.2).sub.2      -- phenyl H H H HCl 285-287 13 H --C(CH.sub.3).sub.2 --(CH.sub.2).sub.2      -- 4-CH.sub.3 -phenyl H H H HCl 272-285 14 H --C(CH.sub.3).sub.2      --(CH.sub.2).sub.2 -- H.sub.2 C═CF-- H H H HCl 249-251 (decomp) 15 H      --C(C.sub.2 H.sub.5).sub.2 --(CH.sub.2).sub.2 -- phenyl H H H HCl      275-277 16 H --C(C.sub.2 H.sub.5).sub.2 --(CH.sub.2).sub.2 -- 4-CH.sub.3      -phenyl H H H HCl 273-274 17 H --C(CH.sub.3).sub.2 --(CH.sub.2).sub.2 --      --CH.sub.2 --CH.sub.2 --CH.sub.2 --CH.sub.2 --CH.sub.2 H H HCl 254-257      18 5-Br --(CH.sub.2).sub.3 -- H H CH.sub.3 H base 106-108 19 5-Br      --(CH.sub.2).sub.3 -- 4-CH.sub.3 -phenyl H H H HCl 307-310 20 5-CF.sub.3      --(CH.sub.2).sub.3 -- 4-CH.sub.3 -phenyl H H H HCl 290.5-291.5 21      5-CF.sub.3 --(CH.sub.2).sub.3 -- 4-CH.sub.3 O-phenyl H H H HCl 286-288      22 5-CF.sub.3 --(CH.sub.2).sub.3 -- 4-HCF.sub.2 --CF.sub.2 O-phenyl H H      H HCl 281-281.5 23 6-F --(CH.sub.2).sub.3 -- phenyl H H H HCl 274-276.5      24 6-F --(CH.sub.2).sub.3 -- 4-CH.sub.3 -phenyl H H H HCl 267 (decomp)      25 6-Cl --(CH.sub.2).sub.3 -- H.sub.2 C═CF-- H H H HCl 145 (decomp)      26 6-Cl --(CH.sub.2).sub.3       -- phenyl H H H HCl 295-297 27 6-Cl --(CH.sub.2).sub.3 -- phenyl H      CH.sub.3 --C═O H base foam 28 6-Cl --(CH.sub.2).sub.3 -- phenyl H      C.sub.2 H.sub.5 --O--C═O H base 193-195 29 6-Cl --(CH.sub.2).sub.3      -- 2-F-phenyl H H H HCl 305-307 30 6-Cl --(CH.sub.2).sub.3       -- 2-Cl-phenyl H H H HCl 317-318 31 6-Cl --(CH.sub.2).sub.3       -- 4-CH.sub.3 -phenyl H H H HCl 275-278 32 6-Cl --(CH.sub.2).sub.3 --      4-CH.sub.3 CH.sub.2 -phenyl H H H HCl 258-262 33 6-Cl --(CH.sub.2).sub.3      -- 4-(CH.sub.3).sub.2       CH--O-phenyl H H H HCl 275-280 34 6-Cl --(CH.sub.2).sub.3       -- 4-HCF.sub.2 --CF.sub.2       --O-phenyl H H H HCl 287-289 35 6-Cl --C(CH.sub.3).sub.2       --(CH.sub.2).sub.2       -- cyclohexyl H H H HCl 269-270 36 6-Cl --C(CH.sub.3).sub.2       --(CH.sub.2).sub.2       -- phenyl H H H HCl 280.281 37 6-Cl --C(CH.sub.3).sub.2       --(CH.sub.2).sub.2 -- 4-CH.sub.3       -phenyl H H H base 104-105 38 6-CH.sub.3 --(CH.sub.2).sub.3 -- phenyl H      H H HCl 287-289 39 6-CH.sub.3 --(CH.sub.2).sub.3 -- 4-CH.sub.3 -phenyl H      H H HCl 291-295 40 6-CH.sub.3 --C(CH.sub.3).sub.2 --(CH.sub.2).sub.2 --      4-CH.sub.3 -phenyl H H H HCl 278-280 41 6-(CH.sub.3).sub.2       CH-- --C(CH.sub.3).sub.2 --(CH.sub.2).sub.2 -- phenyl H H H HCl 200-260      42 6-(CH.sub.3).sub.2 CH-- --C(CH.sub.3).sub.2 --(CH.sub.2).sub.2 --      4-CH.sub.3 -phenyl H H H HCl 170-210 43 7-F --(CH.sub.2).sub.3 -- H H H      H HCl 279-280 44 7-F --(CH.sub.2).sub.3 -- cyclohexyl H H H HCl 275-278      45 H --(CH.sub.2).sub.4 -- 4-CH.sub.3 -phenyl H H H HCl 262-266 46 H      --(CH.sub.2).sub.4 -- 4-CH.sub. 3 -phenyl CH.sub.3 --C═O H H base      79-85 47 H --(CH.sub.2).sub.4 -- 4-CH.sub.3 CH.sub.2 -phenyl H H H HCl      278-280 48 H --(CH.sub.2).sub.4 -- 4-(CH.sub.3).sub.2 CH-phenyl H H H      HCl 273-375 49 H --(CH.sub.2).sub.4 -- 4-(CH.sub.3).sub.2 N-phenyl H H H      2HCl 216-218 50 H --(CH.sub.2).sub.4 -- 4-HCF.sub.2 --CF.sub.2       --O-phenyl H H H HCl 241-242.2 51 H --(CH.sub.2).sub.4       -- 2,4-diCH.sub.3 -phenyl H H H HCl 266.8-270.2 52 H --(CH.sub.2).sub.4      -- 3,5-diCH.sub.3 -4-CH.sub.3       O-phenyl H H H HCl 278-281 53 H --(CH.sub.2).sub.4 -- H.sub.2       C═CF-- H H H HCl 222-225 54 6-CH.sub.3 --(CH.sub.2).sub.4 -- phenyl      H H H base oil 55 6-CH.sub.3 --(CH.sub.2).sub.4 -- 4-CH.sub.3 -phenyl H      H H base oil 56 H --(CH.sub.2).sub.5 -- 4-CH.sub.3 -phenyl H H H HCl      267.5-270.5 57 H --(CH.sub.2).sub.5 -- 4-CF.sub.3 H H H HCl 216-217 58 H      --(CH.sub.2).sub.5       -- cyclopentyl H H H HCl 271-272 59 H --(CH.sub.2).sub.5 -- isopropyl H      H H HCl 253-254 60 H --(CH.sub.2).sub.5 -- H.sub.2 C═CF-- H H H HCl      223-224      *mixture of cis:trans of 4:1

EXAMPLE II (R,S)-8-Cyclohexyl-5,6,8,9,10,11-hexahydro-4H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-ij]-quinoline hydrochloride

a) 0.5 g (1.61 mmol) of 1-[2-(cyclohexyl-carboxamido)-ethyl]-5,6-dihydro-4H-pyrrolo-[3,2,1-ij]-quinoline were mixed with 6 ml of phosphoroxy chloride and stirred for 1 hour at 105° C. The excess of phosphoroxy chloride was then distilled off in vacuo. The residue was extracted with methylene chloride and excess of 2N sodium hydroxide. The organic layer was dried and evaporated in vacuo. The residue was dissolved in ethyl acetate, and 1.1 equivalent of hydrochloric acid in ethanol was added. The solid substance was sucked off, washed with ethyl acetate and dried. Yield 0.45 g (85%) of 8-cyclohexyl-5,6,10,11-tetrahydro-4H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-ij]-quinoline hydrochloride having a melting point of 193°-195° C.

b) 0.45 g (1.37 mmol) of the hydrochloride compound obtained in a) were dissolved in 30 ml of methanol and hydrogenated with platinum as a catalyst at room temperature. After 30 min. of hydrogenating the reaction mixture was sucked off. The filtrate was evaporated in vacuo. The residue was washed with ethyl acetate and dried. Yield: 0.44 g (97%) of (R,S)-8-cyclohexyl-5,6,8,9,10,11-hexahydro-4H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-ij]-quinoline hydrochloride having a melting point of 273°-275° C.

In an analogous way the following compound was obtained: (R,S)-1-cycloheptyl-1,2,3,4,9,10,11,12-octahydro-8H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-kl]-benzazocine hydrochloride; melting point 258°-258.5° C.

EXAMPLE III 2-Chloro-9-ethoxycarbonyl-8-phenyl-5,6,8,9,10,11-hexahydro-4H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-ij]-quinolinehydrochoride

2.26 g (7 mmol) Of 2-chloro-8-phenyl-5,6,8,9,10,11-hexahydro-4H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-ij]-quinoline were dissolved in 30 ml of methylene chloride. 0.95 ml Of triethylamine and 0.66 ml of chloroformic acid ethyl ester were added successively at 20° C. and the mixture was stirred at 20° C. for two hours. The mixture was then washed with 2N hydrochloric acid and the organic layer was evaporated in vacuo. The residue was chromatogrpahed over silicagel using methanol/methylene chloride 3:97 as an eluent.

Yield 2.3 g (=93%), melting-point 193°-195° C.

EXAMPLE IV (-)-1-Cyclohexyl-1,2,3,4,9,10,11,12-octahydro-4H-pyrido[4',3':4,5]-pyrrolo-[3,2,1-kl]-[1]-benzazocine hydrochloride

8.0 g (0.025 mol) (R,S)-1-cyclohexyl-1,2,3,4,9,10,11,12-octahydro-8H-pyrido-[4',3':4,5]-pyrrolo-[3,2,1-kl]-[1]-benzazocine were dissolved in 240 ml of warm methyl ethyl ketone. 4.35 g (0.015 mol)of (-)-diacetone-2-keto-L-gulonic acid, dissolved in 60 ml of warm methyl ethyl ketone, were added and the mixture was cooled. The solid substance was sucked off after a few hours, washed with little methyl ethyl ketone and dried. The obtained salt was extracted with excess of 2N sodium hydroxide and methylene chloride. The organic layer was evaporated in vacuo. The residue was dissolved in 150 ml of warm methyl ethyl ketone. A solution of 2.95 g (0.01 mol) of (-)-diacetone-2-keto-L-gulonic acid in 50 ml of warm methyl ethyl ketone was added, the mixture was cooled, and after a few hours it was sucked off, washed and dried. The obtained salt was extracted with 2N sodium hydroxide and methylene chloride. The organic layer was dried and evaporated in vacuo. The residue was dissolved in ethyl acetate, and 1.1 equivalent of hydrochloric acid in ethanol was added. The solid substance was sucked off, washed with ethyl acetate and dried. Yield: 3.2 g; [α]_(D) ²⁵ =-73.3° (c=2.0; methanol); melting point: decomposition starts at 240° C. According to HPLC-analysis at least 98% of the product is the levo rotating isomer.

EXAMPLE V (+)-1-Cyclohexyl-1,2,3,4,9,10,11,12-octahydro-8H-pyrido-[4',3':4,5]-pyrrolo[3,2,1-kl]-[1]-benzazocine hydrochloride

The mother liquors obtained in Example IV after sucking off the (-)-diacetone-2-keto-L-gulonic acid salts were added to each other and evaporated in vacuo. The residue was extracted with 2N sodium hydroxide and methylene chloride, and evaporated in vacuo. The residue was dissolved in 150 ml of warm methyl ethyl ketone. 1.95 g of D(-)-mandelic acid dissolved in 50 ml of warm methyl ethyl ketone were added, and the mixture was left to cool down. After a few hours the mixture was sucked off, washed with methyl ethyl ketone and dried. The obtained salt was extracted with methylene chloride and excess of 2N sodium hydroxide. The organic layer was dried and evaporated in vacuo. The residue was dissolved in ethyl acetate, and 1.1 equivalent of hydrochloric acid in ethanol was added. The solid substance was sucked off, washed with ethyl acetate and dried.

Yield: 3.5 g; [α]_(D) ²⁵ =+68.4° (C=2.0; methanol). Melting point: decomposition starts at 240° C. According to HPLC-analysis at least 98% of the product consists of the dextro rotating isomer. 

We claim:
 1. A compound of formula 1: ##STR9## wherein R₁ is straight or branched alkyl having 1-4 C-atoms, halogen or trifluoromethyl;R₂ +R₃ together with the carbon atom and the nitrogen atom to which they are linked, and the intermediate carbon atom, form a heterocyclic 7-membered ring, which may be substituted with 1 or 2 alkyl groups having 1-2 C-atoms; R₄ is hydrogen, straight or branched alkyl having 1-4 C-atoms, trifluoromethyl, alkenyl or halogenated alkenyl having 2-4 C-atoms, cycloalkyl having 3-6 C-atoms, phenyl, benzyl, tolyl or methoxyphenyl in which groups the phenyl ring may be halogenated; R₅, R₆ and R₇ are hydrogen; and pharmaceutically acceptable salts and prodrugs thereof.
 2. A pharmaceutical composition having fibrinolytic activity comprising an effective amount of a compound as claimed in claim 1 in admixture with a pharmaceutically acceptable carrier.
 3. A method of dissolving blood clots which comprises administering to a patient in need of said treatment, a fibrinolytically effective amount of a compound of claim
 1. 